Figure 1.

Segregation of two copies of the transgene linked in cis (A) or trans (B). K₁^R and K₂^R indicate transgenic loci whereas K₁^S and K₂^S indicate absence of the transgene on the chromosome. [K^R] and [K^S] are the kanamycin-resistant and kanamycin-sensitive phenotypes, respectively. r - recombination frequency between the linked inserts. Gametes T0 are gametes produced by T0 transformed plants. When r ≥ 0.5, the two transgenes segregate independently while for r < 0.5 the two transgenes recombined with a frequency (r) equal to the distance separating the physically linked inserts.