Table 3.
Absence of selectiona
|
Presence of selectionb
|
|||||||
---|---|---|---|---|---|---|---|---|
KR//KR | KR//KS | KS//KS | Segregation ratio | KR//KR | KR//KS | KS//KS | Segregation ratio | |
T0 | - | 1 | - | 1:0 | - | 1 | - | 1:0 |
T1 | 1/4 | 1/2 | 1/4 | 3:1 | 1/4 | 1/2 | 1/4 | 3:1 |
T2 | 3/8 | 2/8 | 3/8 | 5:3 | 3/6 | 2/6 | 1/6 | 5:1 |
T0 individuals were heterozygous for one copy of the transgene.
Plants germinated on non-selective medium without kanamycin,
Plants germinated on selective medium with kanamycin, KR//KR: kanamycin-resistant homozygous genotype, KR//KS: kanamycin-resistant heterozygous genotype and Ks//KS: kanamycin-sensitive homozygous genotype.