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. 2012 Jul 16;21(20):4431–4447. doi: 10.1093/hmg/dds285

Figure 5.

Figure 5.

NMJs from Smn1C/C mutant display a reduction in synaptic efficacy. Intracellular recordings were performed in the splenius capitis muscle at PND90 and PND350. Quantification of synaptic potentials and efficacy: (A) MEPP amplitude (PND90: Smn1+/+ 0.38 ± 0.02 versus Smn1C/C 0.44 ± 0.02 mV, P < 0.05; PND350: Smn1+/+ 0.37 ± 0.02 versus Smn1C/C 0.43 ± 0.02 mV, P < 0.05). (B) MEPP frequency (PND90: Smn1+/+ 1.76 ± 0.17 versus Smn1C/C 2.08 ± 0.15 Hz, P = 0.15; PND350: Smn1+/+ 1.89 ± 0.22 versus Smn1C/C 1.85 ± 0.14 Hz, P = 0.88). (C) EPP amplitude (PND90: Smn1+/+ 27.50 ± 1.55 versus Smn1C/C 26.65 ± 1.27 mV, P = 0.67; PND350: Smn1+/+ 25.09 ± 1.05 versus Smn1C/C 24.72 ± 1.45 mV, P = 0.84). (D) Quantal content (PND90: Smn1+/+ 59.35 ± 3.06 versus Smn1C/C 50.96 ± 2.42, P < 0.05; PND350: Smn1+/+ 54.08 ± 1.98 versus Smn1C/C 47.59 ± 2.43, P < 0.05). There is an increase in the amplitude of MEPPs and a decrease in the quantal content in Smn1C/C NMJs. (n = 40–60 NMJs from four animals in each genotype). Error bars show SEM.