Figure 6.

Effect of MyD88 and TIRAP inhibitors on TLR2 modulation of IFN-γ production. Spleen T cells (5 × 10⁶ cells/mL), collected from sham vehicle (white bars) and burn ethanol (black bars) groups, were cultured with plate-bound anti-CD3 (2 μg/mL) in the presence or absence of TLR2 agonist (HKLM 10⁸ cells/mL), and MyD88 inhibitory peptide, TIRAP inhibitory peptide or control peptides, using doses of 25 μmol/L (A), 50 μmol/L (B) and 100 μmol/L (C) for 48 h. Supernatants were collected to determine IFN-γ production. Values are means ± SEM from four to eight animals/group. *P < 0.05 compared with corresponding sham vehicle group; ^†P < 0.05 compared with respective αCD3 + TLR2 group; ^‡P < 0.05 compared with respective αCD3 alone.