Figure 7.

Effect of MyD88 and TIRAP inhibitors on TLR2 modulation of p38 phosphorylation. Spleen T cells (10⁷ cells/mL), collected from various experimental groups, were stimulated with anti-CD3 for 10 min and lysed. Lysates were analyzed for phoso-p38 by Western blot. Blots were stripped and reprobed for p38 (A). T cells (10⁷ cells/mL) were pretreated with 25 μmol/L MyD88 inhibitory peptide or TIRAP inhibitory peptide for 3 h, and treated with TLR2 agonist (HKLM 10⁸ cells/mL) for 5 min following CD3 stimulation for 10 min (1, αCD3; 2, αCD3 + TLR2 agonist; 3, αCD3 + TLR2 agonist + MyD88 inhibitor; 4, αCD3 + TLR2 agonist + TIRAP inhibitor). T cells were lysed and phosphorylation of p38 was determined. Blots were stripped and reprobed for p38 protein levels and β-actin (B). Densitometric values for phosphorylation were normalized to β-actin and are shown in (C) as means ± SEM from three independent animal pools, each pool contained three to four animals; white bars indicate sham vehicle, black bars indicate burn ethanol. *P < 0.05 compared with αCD3 alone and other respective groups.