Figure 9.

Effect of p38 and ERK inhibitors on TLR2 modulation of IFN-γ production. Spleen T cells (5 × 10⁶ cells/mL) collected from sham vehicle (white bars) and burn ethanol (black bars), were cultured with (A) or without (B) plate-bound anti-CD3 (2 μg/mL) in the presence or absence of TLR2 agonist (HKLM 10⁸ cells/mL), p38 inhibitor (SB 203580 10 μmol/L) and/or ERK inhibitor (PD 98059 50 μmol/L) for 48 h. Supernatants were collected and analyzed for IFN-γ production. Values are means ± SEM from four to six animals/group. *P < 0.05 compared with αCD3 sham vehicle. ^†P < 0.05 compared with other groups.