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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Trends Biochem Sci. 2012 Aug 30;37(10):411–417. doi: 10.1016/j.tibs.2012.07.004

Figure 3. The pre-targeting steps of the mammalian GET pathway.

Figure 3

Bag6 (a mammalian protein with no apparent yeast homolog) scaffolds the formation of a stable ternary complex with Ubl4a and TRC35 (the mammalian homologs of Get5 and Get4, respectively). The TMD (yellow) in the exit tunnel somehow recruits this complex to the ribosome to presumably minimize competing off-GET pathway interactions. The Bag6 complex can handoff substrates to TRC40 (the mammalian Get3 homolog) while still associated with the ribosome. SGTA (the mammalian Sgt2 homolog) is shown as a transparent shape to de-emphasize its role, but it probably also recognizes newly synthesized TA proteins and delivers them to TRC40 by dynamically associating with the ternary Ubl4a-Bag6-TRC35 complex.