Table 1.
Induction of EAE following adoptive transfer of MOG specific TH17 cells and active immunization.
| Mice | Groups | Disease incidence (%) |
Mean score α |
Mean day of onsetα |
Mortality (%) |
Statistics | |
|---|---|---|---|---|---|---|---|
| 2D2 | IL-1β+IL-6+IL-23 | 20/21 (95%) | 3.7±0.29 | 12.2±0.35 | 10/21 (48%) | ns | |
| 2D2 | TGF-β3+IL-6 | 13/15 (86%) | 3.5±0.35 | 12.7±0.92 | 4/15 (26%) | p<0.001 | |
| 2D2 | TGF-β1+IL-6 | 10/17 (58%) | 2.6±0.45 | 14.1±1.77 | 0/17 (0%) | ||
| 2D2 | TGF-β1+IL-6+IL-23 | 11/21 (52%) | 3.6±1.53 | 14.8±2.40 | 5/21 (23%) | p<0.001χ | |
| 2D2xIL-23R−/− | IL-1β+IL-6+IL-23 | 0/8 (0%) | n/a | n/a | 0/8 (0%) | p<0.001β | |
| 2D2xIL-23R−/− | TGF-β3+IL-6 | 0/8 (0%) | n/a | n/a | 0/8 (0%) | p<0.001β | |
| Wt | Isotype control | 19/22 (86%) | 2.4±0.25 | 9±0.83 | 0/22 (0%) | p<0.001 | |
| Wt | Anti-TGF-β3 | 710 (70%) | 1.6±0.37 | 9.8±2.2 | 0/10 (0%) | ||
| 2D2xTbx21−/− | TGF-β3+IL-6 | 8/11 (73%) | 2.8±1.13 | 18.9±4.58 | 1/11 (1%) | p<0.001 | |
| 2D2xTbx21 | TGF-β1+IL-6 | 0/11 (0%) | n/a | n/a | 0/11 (0%) | ||
| 2D2xTbx21 | TGF-β1+IL-6+IL-23 | 0/7 (0%) | n/a | n/a | 0/7 (0%) | p<0.001β | |
The transferred 2D2 CD4+ cells were cultured in the presence of various combinations of IL-23, TGF-β, IL-1β, and IL-6.
Active EAE was induced with MOG35-55/CFA plus pertussis toxin.
Disease incidence, mean day of onset, and mortality are reported as number of mice affected/total number of mice
α
Mean calculated only from mice that developed clinical signs of experimental autoimmune encephaltomyelitis.
β
Compared to 2D2 controls with indicated cytokine conditions
χ
Compared to 2D2 (TGF-β1+IL-6+IL-23) to 2D2 (TGF-β1+IL-6) conditions
n/a : Denotes: not applicable
ns: Denotes: not significant