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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 13;189(6):3178–3187. doi: 10.4049/jimmunol.1201053

FIGURE 2.

FIGURE 2

P. gingivalis carriage state and frequency of blood mDCs, before and after induced bacteremia. A. Image series from scanning laser confocal microscopy (panels 1–7, z-stack series of 1μm slices) of CD1c+ (BDCA-1+) blood mDCs from oral carriage positive CP patient, permeablized and cytocentrifuged on slides and probed with AEZαMfa1-PE, followed by Vectashield ™ mounting media. P. gingivalis (red) in mDC is shown by white arrow. B. Epifluorescence deconvolution image analysis of blood mDCs from a healthy donor pulsed in vitro with CFSE-labeled P. gingivalis 381 (green) at an MOI of 1 for 3 hours. MDCs were counterstained with DAPI (blue) and RPE-conjugated DC-SIGN (red). C. Significant increase in P. gingivalis 16s rDNA content of blood mDCs from CP subjects (n=6) 24 hours after local debridement (S&RP). MDCs were analyzed for 16s rDNA of P. gingivalis, quantified as in the supplementary methods. (*p=0.02, paired T-test). D. Representative scattergrams from flow cytometry analysis of CD1c+ (BDCA-1+), CD209 (DC-SIGN+) blood mDCs before (0hr) and 24 hours after local debridement (S&RP) of CP patients as described in materials and methods. E. Significant increase in mean number (panel 1) and percentages (panel 2) of blood mDCs 24 hours after S&RP (per 30,000 PBMCs) (**p<0.05, Paired t-test).