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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: J Immunol. 2012 Aug 13;189(6):3178–3187. doi: 10.4049/jimmunol.1201053

FIGURE 5.

FIGURE 5

P. gingivalis infected mDCs in atherosclerotic plaque of CP patient A. Representative post- mortem coronary artery 7μM tissue section from ACS/CP patient stained with H&E, image obtained with enhanced light microscopy (Nikon E600) (4x). B. CAD tissue sections were stained with FITC-conjugated CD1c+, C. RPE-conjugated DC-SIGN+ and D. DC-SIGN-mfa-1 dual staining using FITC-conjugated DC-SIGN+ and Alexa Fluor 594 conjugated-mfa-1. Prior to staining, all tissue sections were blocked with 5% BSA in PBS, along with anti-human FcR block reagent. Appropriate controls included isotype matched antibodies and pre-immune antibodies (not shown). All sections were mounted with VectaShield mounting medium containing DAPI. Images were acquired with a Zeiss LSM 510 META NLO Two-Photon Laser Scanning Confocal Microscope System.