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(A) Diagram of LMWP-SOD1 conjugates. LMWP was chemically conjugated to the amine group of SOD1 via SMCC. (B) Analysis, detection, and purification of LMWP-SOD1 using a heparin affinity column. (C) SOD1 and LMWP-SOD1 were confirmed by Coomassie staining. Lane 1 is the size marker, lane 2 represents SOD1, and lane 3 represents LMWP-SOD1.
Abbreviations: LMWP, low molecular weight protamine; SOD1, superoxide dismutase; SMCC, succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate.
