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Immunoblotting in carriers 1 and 8 and Coomassie staining for protein quantification by densitometric analysis. A) Immunoblot with DYS2 antibody (directed against carboxy terminal region of dystrophin) shows protein levels of 15% for carrier 1 and 70% for carrier 8, with respect to control. B) Immunoblot with DYS1 antibody (directed against rod domain) reveals 11% and 87% of normal protein levels for carriers 1 and 8, respectively.
