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. Author manuscript; available in PMC: 2012 Sep 27.
Published in final edited form as: Nat Immunol. 2010 Aug 1;11(9):827–835. doi: 10.1038/ni.1913

Figure 7.

Figure 7

Most trout gut luminal bacteria are predominantly coated with IgT. (a,b) Staining of propidium iodide–positive trout gut lumen bacteria (3 × 104) with anti-IgT (a; solid lines) or anti-IgM (b; solid lines) or their respective isotype-matched control antibodies (shaded histograms). (c) Coating of bacteria with IgT or IgM. Each symbol represents an individual fish (n = 17); red horizontal lines indicate the median. P value, nonparametric Mann–Whitney test. (d–g) Differential interference contrast images of luminal bacteria stained with a DAPI-Hoeschst solution (blue; d), anti-IgM (red; e) or anti-IgT (green; f), or merged IgT and IgM staining (orange; g). Isotype-matched control antibody staining, Supplementary Figure 5f,g. Orange arrows indicate bacteria stained for both IgT and IgM. Scale bar, 5 µm. (h) Immunoblot analysis of IgT and IgM on luminal bacteria: lane 1, 0.2 µg purified IgM or IgT; lanes 2–6, luminal bacteria (n = 5 fish). Data are representative of at least five independent experiments.