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A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) Nrac-GFP and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.
