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. 2012 Sep 27;6(9):e1823. doi: 10.1371/journal.pntd.0001823

Figure 2. Growth of wtBUNV and rBUNdelNSs2 in Aedes albopictus cell lines.

Figure 2

A. Growth curves. C6/36, C7-10 and U4.4 cells were infected at an MOI of 1 PFU/cell. At the indicated time points, supernatants were collected and viral titres were determined by plaque assay. B. Viral N protein expression. Cells were infected at an MOI of 1 and at the indicated time points (hpi), cells were lysed, and proteins separated by 12% SDS-PAGE. The proteins were transferred to a membrane and incubated with anti-BUNV N protein (N) or anti-tubulin (T) antibodies.