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. 2012 Sep 27;6(9):e1823. doi: 10.1371/journal.pntd.0001823

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© 2012 Szemiel et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mosquito cells were infected with rBUN-GcGFP or rBUNdelNSs-GcGFP at an MOI of 3 PFU/cell. The results present cells stained with anti-BUNN/anti-rabbit TexasRed (red signal), and with anti-tubulin/anti-mouse CY5 (light blue signal). The green signal shows autofluorescence of GFP-tagged Gc and the blue signal is DAPI staining of nuclei. A. C6/36 cell infected with rBUN-GcGFP virus during the acute stage of infection. B. C6/36, C7-10 and U4.4 cells were infected with fluorescent viruses. Three morphological stages of infection are presented: early, acute, and late. Only the merged images are shown.