Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 1;23(19):3882–3898. doi: 10.1091/mbc.E12-05-0337

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Gupta and Dey. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 1: — Effect of PTEN or SHIP2 down-regulation on impaired Akt and GSK3β phosphorylation. Scrambled or PTEN- or SHIP2-specific siRNA–transfected N2A cells were differentiated in the absence (MF) or chronic presence (MFI) of insulin for 3 d and stimulated with or without insulin (100 nM) for 30 min. (A) Cell lysates were subjected to Western immunoblotting and probed with anti-PTEN (panel A), anti-SHIP2 (panel B), anti-actin (panel C), anti–phospho-Akt (Ser-473; panel D), anti-Akt (panel E), anti-phospho GSK3β (Ser-9; panel F), or anti-GSK3β (panel G) antibodies. (B) Bar diagram representing relative densitometric values of PTEN expression after normalizing with actin expression (PTEN/actin). (C) Bar diagram representing relative densitometric values of SHIP2 expression after normalizing with actin expression (SHIP2/actin). (D) Bar diagram representing relative densitometric values of pAkt (Ser-473) after normalizing with Akt expression (pAkt/Akt). (E) Bar diagram representing relative densitometric values of pGSK3β (Ser-9) after normalizing with GSK3β expression (pGSK3β/GSK3β). All the experiments were repeated thrice, and a representative result is shown. Values are mean ± SE. **p < 0.01 compared with lane 1; ^$$p < 0.01 compared with lane 2; ^##p < 0.01 compared with lane 3; ^θθp < 0.01 compared with lane 4. Open bars, MF; solid bars, MFI. IB, immunoblotted.