Figure 3. Relative correlation factor by distance in fully-matched TtAgo ternary complex.

For clarity, the correlation factor for each distance shell is normalized by its value in guide strand position 1. Note that cleavage site residues have strong effect on nearby protein residues, but as distance from the nucleotide is increased, this effect is decreased, and the effect of nucleotides 3–6 becomes relatively more prominent. This suggests a long-range effect on internal allosteric pathways of the seed region, but only a local effect of the active site. Similar results are obtained for all mismatched ternary complexes. Distance is in Ångstroms.