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. 2012 Sep 27;7(9):e46149. doi: 10.1371/journal.pone.0046149

Figure 4. Silibinin reverses CEES-induced oxidative stress in skin cells.

Figure 4

Mouse epidermal JB6 cells (A and B) and fibroblasts (C) were seeded (120,000 cells/plate) and grown overnight in 60 mm petri dishes. The cells were then exposed to DMSO or 0.5 mM CEES in DMSO, or treated with either 10 µM silibinin alone or with silibinin following 30 min CEES exposure for 4–6 h. The cells were then incubated for 30 min or 1 h with DHE (A and C) or MitoSOX Red (B), respectively, and the live cell fluorescence was determined using flowcytometry as described under Materials and Methods. Representative pictures of the flow cytograms of the epidermal JB6 cells (A and B) and dermal fibroblasts (C) are shown on the left side of the graphical presentation of the quantitative data. Data shown are mean ± SEM of three independent samples. *, p<0.05 as compared to CEES exposed group. VC, vehicle (DMSO) control; SB, silibinin; CEES+SB, silibinin treatment 30 min after CEES exposure; NS, not significant.