Table 2.
Kinetic rates of VIP36-SP-GFP recycling between the Golgi and ER
| Experiment | Transport rate
constants (% · min−1)
|
t1/2a (min)
|
Exchange
timea (min)
|
|||
|---|---|---|---|---|---|---|
| ER→Golgi | Golgi→ER | ER→Golgi | Golgi→ER | ER→Golgi | Golgi→ER | |
| ER bleach (n = 10) | 1.22b ± 0.66 | 7.60c ± 2.24 | 113 ± 70 | 1.36 ± 0.45 | 178 ± 106 | 17.3 ± 5.0 |
| Golgi bleach (n = 9) | 1.23c ± 0.59 | 7.74b ± 3.14 | 98.1 ± 40.4 | 1.94 ± 0.75 | 160 ± 66 | 22.2 ± 8.6 |
| Combined | 1.22 ± 0.44 | 7.68 ± 1.94 | 105 ± 39 | 1.67 ± 0.45 | 169 ± 59 | 19.9 ± 5.1 |
Intervals are 90% confidence for the number of trials shown.
a
Calculated for each experiment and then averaged.
b
Transport rates calculated from the fitted curves and steady-state fraction (see MATERIALS AND METHODS).
c
Transport rates derived directly from the fitted curve.