Table 3.
Comparison of VIP36-SP-FP with published kinetic parameters for proteins along the secretory pathway
| Protein | Linear, first order rate
constants (% · min−1)
|
Exchange
timea (min)
|
||||
|---|---|---|---|---|---|---|
| ER→Golgi | Golgi→ER | Golgi→PM | ER | Golgi | PM | |
| VIP36-SP-GFP | 1.22 | 7.68 | n.d. | 169.0 | 19.9 | n.d. |
| GalTase-GFP3b | 3.60 | 1.80 | n.d. | 27.5 | 57.2 | n.d. |
| VSVG-GFPc | 2.84 | n.d. | 3.0 | 39.4 | 42.8 | 709 |
n.d., not detected.
a
Exchange times for VIP36-SP-FP were calculated for each experiment and then averaged.
b
Golgi type II resident protein (Zaal et al., 1999).
c
Secretory cargo (Hirschberg et al., 1998).