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. 2012 Sep 27;7(9):e46405. doi: 10.1371/journal.pone.0046405

Figure 5. Loss of synaptic activity in serotonergic neurons does not affect cell numbers in the adult brain.

Figure 5

A) Immunohistochemistry of the adult brain expressing mCD8GFP/TNTvif in TRHGAL4 domains. All TRHGAL4 positive neurons (anti-GFP, green) stain with anti-5-HT (red and green merge), except for 6 medial cells (MD), 1 cell in LP1 (LG1), 2 in LP2 (LG2), 1 in SE1 (SG1) and 1 in SE3 (SE3), which are GFP-positive but 5-HT negative. B) Immunohistochemistry on a TRHGAL4/TNTH brain collected from flies which passed the column flight test (fliers). Medial cells are seen (green). C) Immunohistochemistry on a TRH/TNTH brain collected from non-fliers. No difference is seen as compared with controls in A and B. D) Schematic of the brain showing cells marked by TRHGAL4 (anti-GFP, green) and anti-5-HT. Medial cells are not stained with anti-5-HT. E) Number of cells marked by anti-GFP and anti-5-HT staining does not vary between control and tetanus toxin expressing serotonergic neurons among fliers and non-fliers. (Nomenclature based on [26]).