Figure 1. Effect of HD5 on HIV-1 replication in purified CD4⁺ T lymphocytes.

(A) Effect of recombinant HD5 on infection by primary isolate HIV-1_J176 in cell-free infection assays performed using the spinoculation method. HIV-1 virions were preincubated for 1 hour at 37°C with HD5 at the indicated concentrations in either RPMI-10 (10%FBS), RPMI-0.3-ITS (no serum) or 10 mM phosphate buffer 0.3-ITS (low salt). Gag p24 concentrations in the extracellular supernatant were measured on day 6 post-infection. Neutralizing mAbs directed to gp120 (2G12) or CD4 (Sim4) were used as positive controls to exclude non-specific infection. The data represent mean values (±SD) from 3 experiments each performed in triplicate. (B) Broad-spectrum inhibition of different HIV-1 strains by HD5. Purified CD4⁺ T lymphocytes were infected with two laboratory-adapted strains (HIV-1_IIIB and HIV-1_BaL) or 4 primary clinical isolates (HIV-1_J1005, HIV-1_p36, HIV-1_J6195 and HIV-1_J176) in the presence of HD5 at 1.4 µM in RPMI-0.3-ITS. Gray bars indicate R5 HIV-1 isolates; solid bars X4 isolates; the striped bar a dualtropic isolate. The data represent mean values (±SD) of three separate experiments, each performed in triplicate.