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Competition of HD5 with a panel of anti-gp120 mAbs with various epitope specificity for binding to plastic-immobilized recombinant gp120 in ELISA. The plates were coated with gp120BaL at 2 µg/mL; HD5 at 5.6 µM was added prior to the anti-gp120 mAbs, all used at 5 µg/ml. CD4 binding site (CD4-BS), CD4-induced coreceptor binding site (Coreceptor-BS). The data represent mean values (±SD) from three experiments.
