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. 2012 Sep 27;7(9):e45208. doi: 10.1371/journal.pone.0045208

Figure 5. Effect of HD5 on cell-surface HIV-1 coreceptor expression: induction of CXCR4 downmodulation.

Figure 5

PM1 cells and primary peripheral blood lymphocytes activated with PHA and IL-2 were preincubated for 2 hours with or without HNP1 or HD5 (each at 14.7 µM) in RPMI-ITS without serum; the cells were then stained with 12G5 or 44717.11, two_conformation-dependent mAbs that recognize antigenically distinct populations of CXCR4 [39] and analyzed by flow cytometry. The level of expression was determined by differences between the level of staining with specific mAbs and with the isotype control. The results are expressed as percent reduction of mean fluorescent intensity (MFI) compared to untreated controls. Data are representative of 4 to 6 independent experiments performed with similar results. Error bars show standard deviations from the mean. *, p = 0.032 and **, p = 0.0084. Wilcoxon Mann-Whitney rank-sum test, 2-tailed.