Table 2. Contents of free amino acids and activities of malate dehydrogenase (MDH) and glutamate dehydrogenase (GDH) in tissue homogenates of symbiotic and bleached Isopora palifera with or without nutrient supplementation.
| Treatment | MDH | GDH | Free amino acids | |
| Total | Essential | |||
| (nmole NAD(P)+ min−1mg−1) | (pmole mg−1) | |||
| Symbiotic control | 77±18a (14) | 40±6a (13) | 385±43a (9) | 103±15a (9) |
| Apo-symbiotic host | 86±6a (9) | 20±4b (9) | 213±21b (11) | 58±8b (11) |
| Apo-symbiotic host fed nutrient A | 109±26a (5) | 24±4ab (5) | 372±29a (5) | 94±11a (5) |
| Apo-symbiotic host fed nutrient B | 42±9a (11) | 41±5a (8) | 281±34b (11) | 80±8ab (11) |
| F3,35 = 2.331 P>0.05 | F3,31 = 3.292 P<0.05 | F3,32 = 5.864 P<0.01 | F3,32 = 3.264 P<0.05 | |
Essential amino acids followed the definition applied to the sea anemone Aiptasia pulchella [19]. Enzyme activities were determined as the amount of NAD(P)H (in nmol) converted to NAD(P) by 1 mg of protein in 1 min. Nutrient compositions of A and B are described in “Materials and Methods” and Table 1. Numbers in parentheses represent the number of colony replicates, and means followed by the same letter do not significantly differ at p = 0.05 (Fisher's least significance difference test). Data are the mean±S.E.