Table 3. Contents of free amino acids and activities of malate dehydrogenase (MDH) and glutamate dehydrogenase (GDH) in tissue homogenates of symbiotic and bleached Stylophora pistillata.

Treatment	MDH	GDH	Free amino acids
			Total	Essential
	(nmole NAD(P)+ min−1mg−1)		(pmole mg−1)
Symbiotic control	46±11 (12)	21±1 (12)	250±20 (12)	75±12 (12)
Apo-symbiotic host	91±6 (18)	21±2 (20)	271±11 (21)	95±8 (21)
	F1,28 = 12.948 p<0.001	F1,30 = 0.026 p>0.05	F1,31 = 0.004 p>0.05	F1,31 = 2.231 p>0.05

Essential amino acids followed the definition applied to the sea anemone Aiptasia pulchella [19]. Enzyme activities were determined as the amount of NAD(P)H (in nmol) converted to NAD(P) by 1 mg of protein in 1 min. Numbers in parentheses represent the number of colony replicates, and data are the mean±S.E.