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. 2012 Sep 27;7(9):e44991. doi: 10.1371/journal.pone.0044991

Figure 3. MSRVenv and syncytin-1 stimulation by EBV and EBVgp350 in U87-MG astrocytes.

Figure 3

The levels of MSRVenv and syncytin-1 mRNAs were evaluated by real time RT-PCR as in Figure 2A, and are expressed as mean fold increases over controls (2−ΔΔCt method). A. Expression of MSRVenv and syncytin-1 in astrocytes cultured alone or co-cultured with the EBV-producer B95-8 cells for 24–48 h. B. Effects of 10 (•) or 100 (♦) ng/ml of EBVgp350 on MSRVenv and syncytin-1 mRNA accumulation during time. C. Suppression of EBVgp350 effects by its pre-incubation with anti-EBVgp350 neutralizing antibody. D. Requirement of NF-κB. Expression of MSRVenv and syncytin-1 by U-87MG cells transfected or not with siRNA against the NF-κB p65 subunit and left overnight with/without 10 ng/ml of EBVgp350. E. Western blot assay of protein extracts from aliquots of the same cultures of Figure 3D. The relevant band was recognized by antibody against the NF-κB p65 subunit; the anti-Grb2 antibody was used to control the loading of equal amounts of proteins.