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Representative western immunoblots for σS in TW14359 (WT), TW14359ΔrpoN (ΔrpoN), TW14359ΔfhlA (ΔfhlA), and TW14359ΔntrC (ΔntrC) before (t = 0 min) and 4 min after addition of tetracycline (Tet.). Stationary phase (Stat.) protein extracts were used as a positive control for σS, and TW14359ΔrpoS (ΔrpoS) as a negative control. Equal loading was controlled for by westerns for GroEL.
