Figure 6. Microsynteny analysis of homologous regions related to BnFLC.A3b, BnFRI.A3, and BnCBF.A3.
Collinear gene models are connected by dotted lines. (A) The colored bar in the center represents the portion of chromosome 3 of B. napus that contains the three genes. The blue arrow indicates the opposite orientation of the ‘W’ block. The left-hand box represents a region of the ‘J’ block (BnA3-J) in which BnFLC.A3b and BnCBF.A3 are located, and alignment of conserved gene models of BnC3-J of B. napus, the triplicated regions (BrA3-J, BrA4-J and BrA5-J) of B. rapa, and the homologous regions in A. thaliana and A. lyrata. Red lines connect the collinear gene models in the ‘J’ block next to FLC and CBF homologues. The right-hand box represents a region of the ‘W’ block in which BnFRI.A3 is located, and alignment of conserved gene models in the triplicated regions (BrA2-W, BrA3-W and BrA4-W) of B. rapa, and the homologous regions in A. thaliana and A. lyrata. Red lines connect the FRI homologues and the FRI homologous fragments. (B) Detailed structure of a region in BrA3-J (indicated by a green box in the left-hand box of (a) from the BAC clone KBrH038M21; [35]). Repeat sequences were identified between the homologues of At2g30000 and At2g30020. GF-1 and GF-2 represent two gene fragments homologous to the ‘R’ block genes, At5G10200 and At5G10150, respectively.