Table 1. List of strains and plasmids.
Escherichia coli strains | |||
MG1655 | F-, λ−, ilvG−, rfb-50, rph-1 | [2] | |
AMD052 | MG1655 ΔthyA | This work | |
AMD095 | MG1655 lacZ 2017-2019 GAT→TGA | This work | |
AMD225 | MG1655 allR-FLAG3 | This work | |
VS003 | MG1655 ΔthyA Phigh:lacZ | This work | |
VS004 | MG1655 ΔthyA Pmed:lacZ | This work | |
VS005 | MG1655 ΔthyA Plow:lacZ | This work | |
VS006 | MG1655 ΔthyA Prha:lacZ | This work | |
ETEC strains | |||
H10407 | Wild-type | [13] | |
AY001 | H10407 ΔthyA | This work | |
AY004 | H10407 eslA LexA site mutation | This work | |
AMD248 | H10407 allR-FLAG3 | This work | |
Salmonella enterica serovar Typhimurium strains | |||
14028s | Wild-type | [14] | |
AMD212 | 14028s ΔthyA | This work | |
JJA001 | 14028s ΔoafA | This work | |
BLP006 | 14028s hilD-FLAG3 | This work | |
Plasmids | |||
pKD46 | Encodes λ recombinase system | [10] | |
pKD13 | kan r recombineering template | [10] | |
pGEM-T | T-tailed cloning vector | Promega | |
pAMD001 | pGEM-T-thyA | This work | |
pAMD135 | pGEM-T-FLAG3-thyA-FLAG3 | This work | |
pVS003 | pGEM-T-Phigh-thyA-Phigh | This work | |
pVS004 | pGEM-T-Pmed-thyA-Pmed | This work | |
PVS005 | pGEM-T-Plow-thyA-Plow | This work | |
pVS006 | pGEM-T-Prha-thyA-Prha | This work |