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. 2012 Sep 27;8(9):e1002982. doi: 10.1371/journal.pgen.1002982

Figure 5. Functional analysis for SNP rs12722508.

Figure 5

A: Immunostaining with an antibody against endogenous RUNX1 validates differential binding between the A- and G-allele of rs12722508, input refers to nuclear extract incubated with either rs12722508 allele. B: mRNA levels are reduced upon esiRNA knock-down with mean and s.d. of triplicates; C: changes in firefly luciferase activity in mock-transfection and upon knock-down of TFAP4, RUNX1, CREB and TFAP4. Knock-down of RUNX1 results in an activation with an allele-specific difference (P = 0.016) demonstrating the functional consequence of differential RUNX1 binding between the two alleles of rs12722508.