Table 2. Major protein components in early embryonic state (EES).

Protein description	NCBInr accession no.	Protein length (aa)	Slice no.	emPAI
contig18794:1:101:3|No Annotation*	EZ761369	33	EES1, 3	874.69
contig18438:8:349:2|No Annotation*	EZ761288	113	EES9, 1–24	535.33
contig04531:1:1454:1|similar to Apolipophorins (Q9U943)|Evalue: 7e-11	EZ759740	484	EES5, 1–25	434.48
contig08625:1:110:2|No Annotation	EZ760340	36	EES8, 2–22	275.13
contig10105:1:309:3|No Annotation	EZ760515	102	EES6, 1–23	258.84
contig18673:1:499:2|similar to Major egg antigen (P12812)|Evalue: 2e-05	EZ761340	166	EES9, 1, 3–23	227.19
contig02293:1:648:1|similar to Vitellogenin-6 (P18948)|Evalue: 5e-14	EZ759327	216	EES6, 2–23, 27	219.67
contig06595:1:952:2|similar to Vitellogenin-1 (P87498)|Evalue: 3e-07	EZ760059	317	EES6, 1–25	206.56
contig08235:860:1596:2|No Annotation*#	EZ760287	245	EES12, 3–24	197.64
contig24531:1:590:3|PREDICTED: apolipoprotein B [Danio rerio](XP_694827.3)|Evalue: 2e-05	EZ762575	196	EES5, 1–23	162.83
contig18537:1:1312:2|PREDICTED: similar to apolipoprotein B [Strongylocentrotus purpuratus] (XP_800206.2)|Evalue: 1e-07	EZ761306	437	EES5, 1–22, 24	141.61
contig26443:1:303:1|No Annotation	EZ763018	101	EES8, 1–20	126.67
contig06373:1:1480:1|similar to Vitellogenin-4 (P18947)|Evalue: 9e-11	EZ760037	493	EES6, 1–24	107.82
contig24202:107:1017:2|similar to Apolipoprotein B-100 (P04114)|Evalue: 3e-18	EZ762460	303	EES5, 1–24	106.16
contig13035:1:170:1|No Annotation	EZ760790	56	EES10, 2–21	90.14
contig26295:1:531:2|similar to Vitellogenin-6 (P18948)|Evalue: 4e-16	EZ762991	176	EES6, 1–20	76.29
contig02295:455:757:2|No Annotation	EZ759329	101	EES6, 3–23	72.73
contig19498:1:162:1|No Annotation	EZ761534	53	EES17, 16–18	69.57
contig23734:1:153:2|similar to Transketolase-like protein 2(Q9D4D4)|Evalue: 6e-16	EZ762348	50	EES8, 8–11, 13–16	66.59
contig02294:1:661:1|similar to Vitellogenin-4 (P18947)|Evalue: 5e-11	EZ759328	220	EES6, 4–22	65.94
contig21262:473:652:1|No Annotation	EZ761852	59	EES18, 1, 4, 16–21	60.53
contig08235:1:820:2|No Annotation	EZ760287	272	EES12, 3–21, 23	57.82
contig24360:1:563:3|No Annotation	EZ762516	187	EES6, 3–20	54.9
contig21510:1907:2014:1|similar to 40S ribosomal protein S30(P62861)|Evalue: 3e-05	EZ761888	35	EES20, 1, 21	43.57
contig02022:1:119:3|No Annotation	EZ759264	39	EES13, 3–11, 12–15	41.58
contig28231:1:506:3|No Annotation	EZ763311	168	EES8, 1–17, 20, 21, 23	40.43
contig13522:1:269:3|No Annotation	EZ760825	89	EES6, 1, 3, 5–17, 21	39.96
contig08851:1:613:2|similar to 60S ribosomal protein L7 (O01802)|Evalue: 4e-82	EZ760361	203	EES1, 1–4	39.62
contig20910:105:416:3|similar to Histone H4 (P62799)|Evalue: 9e-40	EZ761805	103	EES20, 1–5, 14–24	37.24
contig03062:1:281:2|No Annotation	EZ759470	93	EES6, 3–22	37.22
contig17982:1:724:2|similar to Actin-5C (P10987)|Evalue: 1e-138	EZ761195	240	EES12, 13	36.13
contig18941:155:934:2|No Annotation	EZ761416	259	EES14, 1, 11–21	35.41
contig02694:1:571:2|No Annotation*	EZ759400	189	EES10, 6–20	34.64
contig24586:1:771:2|similar to Vitellogenin-6 (P18948)|Evalue: 5e-10	EZ762594	256	EES6, 3–20	34.26
contig26339:1:396:1|No Annotation	EZ762998	131	EES15, 14–16	33.54
contig22711:32:1325:2|similar to Elongation factor 1-alpha (P41752)|Evalue: 0.0	EZ762083	431	EES10, 6–21	32.72
contig07785:1:816:1|No Annotation	EZ760233	272	EES6, 2–17	31.07
contig07915:1:1267:2|similar to Heat shock cognate 71 kDa protein(Q90473)|Evalue: 0.0	EZ760247	422	EES8, 7–20	30.43

38 proteins were found as major components in EES, 10 of which belong to the large lipid transporter superfamily. Other members are the heat shock protein family, structural constituent of ribosome and cytoskeleton. In addition, 20 proteins are without annotation. The contig description is indicated with asterisk, in case we found putative candidates by DomainSweep analysis. For one contig DomainSweep analysis delivered a significant candidate (indicated with #). Proteins with annotation are ordered by their biological function.