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. 2012 Sep 27;8(9):e1002986. doi: 10.1371/journal.pgen.1002986

Figure 1. Knockdown of TCF7L2 promotes elevations in blood glucose levels in C57BL/6 mice.

Figure 1

A) Western blot analysis showing protein expression levels of TCF7L2 M, TCF7L2 S, and TCF7L2 E in livers of high-fat diet-fed or normal chow diet-fed (top), and db/db or C57BL/6 mice (bottom). B) 16 h fasting glucose levels (top, left), 6 h fasting glucose levels (top, right), or feeding glucose levels (bottom) from 8-week-old C57BL/6 male mice that were infected with Ad-US (n = 7) or Ad-shTCF7L2 (n = 6). C) Q-PCR analysis showing effects of Ad-US (n = 3) or Ad-shTCF7L2 (n = 4) on hepatic expression of PEPCK, G6Pase, and PGC1α in C57BL/6 mice fasted for 6 h. D) Q-PCR analysis showing effects of Ad-US (n = 4) or Ad-shTCF7L2 (n = 4) on hepatic expression of G6Pase, PEPCK, and Lipin1 in C57BL/6 mice under feeding conditions. E) Western blot analysis showing effects of Ad-shTCF7L2 on insulin signaling pathway in mice. C57BL/6 mice infected with either Ad-US or Ad-shTCF7L2 for 5 days were fasted for 6 h, and then were given a bolus of insulin or saline for 10 min before being sacrificed. Data in B) represent mean ± SEM, and data in C) and D) represent mean ± SD (*;P<0.05, **;P<0.005, ***;P<0.0005, t-test).