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. 2012 Sep 27;8(9):e1002986. doi: 10.1371/journal.pgen.1002986

Figure 2. Ectopic expression of TCF7L2 in the liver alleviates impaired glucose metabolism in high-fat diet-fed mice.

Figure 2

A) 16 h fasting glucose levels from high-fat diet-fed 14-week-old C57BL/6 male mice that were infected with Ad-GFP (n = 10) or Ad-TCF7L2 M adenovirus (n = 7). B) 6 h fasting glucose levels from high-fat diet-fed 14-week-old C57BL/6 male mice that were infected with Ad-GFP (n = 10) or Ad-TCF7L2 M adenovirus (n = 7). C) Plasma TG and NEFA levels from high-fat diet-fed 14-week-old C57BL/6 male mice that were infected with Ad-GFP (n = 10) or Ad-TCF7L2 M adenovirus (n = 7). D) Q-PCR analysis showing effects of Ad-GFP (n = 4) or Ad-TCF7L2 M (n = 4) on hepatic expression of gluconeogenic genes. E) Glucose tolerance test showing effects of TCF7L2 expression on glucose homeostasis (n = 10 for Ad-GFP, and n = 7 for Ad-TCF7L2 M). F) Western blot analysis showing effects of Ad-TCF7L2 on insulin signaling pathway in mice. High-fat diet-fed C57BL/6 mice infected with either Ad-GFP or Ad-TCF7L2 for 5 days were fasted for 6 h, and then were given a bolus of insulin or saline for 10 min before being sacrificed. Data in A–C) and E) represent mean ± SEM, and data in D) represent mean ± SD (*;P<0.05, **;P<0.005, ***;P<0.0005, t-test).