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Conditions of batch growth of the bioluminescent bacteria P. luminescens, in eNB medium for mass production
| No. | Batch | Details (inoculum and vessel) | Medium conditions |
|---|---|---|---|
| I. | Process scale: batch run 500 ml medium in bioreactor (Sartorius A + of 2 l capacity). | 10% Inoculum (15 h old) contained bioluminescence >1.0 × 104 RLU. | 500 ml NB (0.8%) (with 2% lipids and added glucose 0.2%. |
The run was conducted at a temperature of 29 ± 1°C with agitation of 200 rpm. Air flow was maintained at 0.6 vvm
