. 2012 Jun 8;30(3):1237–1252. doi: 10.1007/s11032-012-9711-1

Table 2.

Selected candidate reference genes, primers, annealing temperatures, amplicon lengths, and actual amplification efficiencies

Gene	Primers	Sequence	Annealing temp. (°C)^a	Amplicon length (bp)	E (±SD)^b
ACT11	act11-F3	CCCAAGGCAAACCGAGAGAAG	60	150	1.00 (0.031)
ACT11	act11-R2	GTGGCTCACACCATCACCAG	60	150	1.00 (0.031)
ACT	act-1	GAGAAGATACAGTGTCTGGA	52	231	0.88 (0.073)
ACT	act-2	ATTACCATCGAAATATTAAAAG	52	231	0.88 (0.073)
EF1	EF1-F2	CGGAGCGTGAAAGAGGAAT	62	185	0.99 (0.069)
EF1	EF1-R2	ACCAGCTTCAAAACCACCAG	62	185	0.99 (0.069)
L2	L2-F2	AGGGTTCATAGCCACACCAC	61	100	1.00 (0.064)
L2	L2-R2	CCGAACTGAGAAGCCCCTAC	61	100	1.00 (0.064)
25S	25S-1	ACATTGTCAGGTGGGGAGTT	59	106	0.79 (0.053)
25S	25S-2	CCTTTTGTTCCACACGAGATT	59	106	0.79 (0.053)
TUB	tub-F1	TGTTGCATCCTGGTACTGCT	61	112	0.98 (0.032)
TUB	tub-R1	GGCTTTCTTGCACTGGTACAC	61	112	0.98 (0.032)

^aAs determined by gradient PCR

^bEfficiency of PCR amplification (±standard deviation)