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. 2001 May;12(5):1519–1527. doi: 10.1091/mbc.12.5.1519

Figure 3.

Figure 3

EGFR-dependent MEK/MAPK activation in HaCaT keratinocytes. (A) Effects of mAb 425 and AG1478 on Elk-1 phosphorylation. Cells were treated for 48 h with mAb 425 (10 μg/ml) or AG1478 (10 μM) before protein extraction and analysis. As a control, the effect of MEK inhibitor PD98059 at 5 and 50 μM is shown. The lane labeled MAPK shows phosphorylation of the Elk-1 substrate by recombinant MAPK. (B) Effects of mAb 425 and AG1478 on GSK-3β phosphorylation in HaCaT keratinocytes. Samples were treated as described under A. As a control, the effect of PI-3-kinase inhibitor LY294002 at 5 and 20 μM is shown. (C) Effect of the pharmacological inhibitor of MEK1/2 activity PD98059 on clonogenicity of HaCaT keratinocytes after 48 h of suspension culture determined as described in the legend to Figure 1. For comparison, the effects of mAb 425 and AG1478 are shown.