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. 2001 May;12(5):1519–1527. doi: 10.1091/mbc.12.5.1519

Figure 5.

Figure 5

Effects of caspase inhibitor Ac-DEVD-CHO on clonogenicity (A) and MAPK phosphorylation (B) of HaCaT keratinocytes in suspension culture. Control HaCaT cells were kept in forced suspension in base medium free of exogenous growth factors for the time periods indicated. Parallel cultures were incubated in the presence of Ac-DEVD-CHO at 120 μM. P42/44 MAPK expression and phosphorylation thereof were determined by Western blot analysis as described in the legend to Figure 5. PARP cleavage was assessed by reprobing the blots using an antibody to the p85 PARP cleavage product.