Table 2.
Genes involved in glycogen synthesis and degradation in M. fumariolicum strain SolV and their expression levels.
| Enzyme | Gene name | E.C. number | Accession nr | Identity to V4 ortholog (%)a | Expression(RPKM value)b |
|---|---|---|---|---|---|
| Glycogen synthase | glgA | 2.4.1.21 | Mfum_1010040 | 74 | 306 |
| 1,4-alpha-glucan-branching enzyme | glgB | 2.4.1.18 | Mfum_170041 | 70 | 154 |
| Glucose-1-phosphate adenylyltransferase | glgC | 2.7.7.27 | Mfum_1020013 | 89 | 391 |
| Glycogen phosphorylase | glgP | 2.4.1.1 | Mfum_1020098 | 81 | 656 |
| Mfum_220010 | 86 | 113 | |||
| Mfum_880004 | 88 | 447 | |||
| Glycogen debranching enzyme | glgX | 3.2.1 | Mfum_40003 | 70 | 381 |
| Amylo-alpha 1,6 glucosidase | gdb | 3.2.1.33 | Mfum_200059 | 84 | 256 |
| Glycosyl transferase (group 1) | rfaG | 2.4.1 | Mfum_200060 | 81 | 43 |
| Phosphoglucomutase | pgm | 5.4.4.2 | Mfum_550015 | 73 | 398 |
aComparison with proteins encoded in the genome of Methylacidiphilum infernorum V4.
bmRNA expression in exponentially growing cells as determined by RNA-seq is expressed as RPKM (Mortazavi et al., 2008).