EGFR genotyping and linkage analysis for chromosome 7 with SimWalk2. A, the EGFR-T790M mutation was not observed in familial lung cancer blood DNA, BAC tumors and normals, or fresh-frozen resected tumors. B, the genetic position for EGFR is at ~73 cM (based on interpolation), between markers D7S1818 (70 cM) and D7S3046 (79 cM). Simwalk2 analysis was based on the parametric method using the genetic model reported by Moscatello et al. (18). Fifty-five pedigrees with five or more affected members were used. C and D, two representative pedigrees of GELCC collected families with predisposition to lung cancer. In (C) and (D), ●. (females) or ■ (males) represent lung, throat, or laryngeal cancer. Numbers below each individuals, sample numbers; numbers in brackets, individual ages at the time the pedigree was constructed; slashes, dead individuals.