Figure 1.
Effect of hyponutrition on cell proliferation and SA-β-gal-positive senescence. GL261 cells (1x106/dish) were seeded into 60-mm dishes and cultured in DMEM supplemented with FBS at concentrations of 1, 2 and 10%, and cultivated for 72 h. (A) Number of viable cells over time. (B) Percentage of SA-β-gal-positive cells according to FBS concentration. *P-value of <0.05. (C) Cytochemical staining for SA-β-gal activity of GL261 cells after cultivation for 72 h. Microphotograph shows positive (blue) cells.