Effects of Endostar and β-elemene on the expression of VEGF and MMP-2 proteins, as well as VEGF mRNA in ascites tumor cells. (A) Cell lysates were collected for western blot analysis of VEGF and MMP-2, and β-actin was used as a loading control. (C and D) Optical densities of VEGF, MMP-2 and β-actin were analyzed and normalized with loading control. (B) VEGF mRNA levels were analyzed by real time qPCR. *P<0.05 vs. control group; #P<0.05 vs. ES (Endostar) 8 mg/kg group; +P<0.05 vs. EL (β-elemene) 100 mg/kg group, n=3. ES, Endostar; EL, β-elemene.