Figure 1.

Calbindin-D_28k expression in mouse incisor. Calbindin-D_28k protein was comparatively immunodetected in the incisors of 14-day-old Msx2^+/+ (A, F), Msx2^+/− (B, G), and Msx2^−/− (C, H) mice. Tissue sections (in ameloblast secretion stage) were fixed in 4% paraformaldehyde, decalcified in ethylenediaminetetraacetate, and embedded in paraffin. Then, 8-µm sections were analyzed by immunohistochemistry with rabbit anti–calbindin-D_28k or rabbit anti–β-galactosidase polyclonal antibodies (for Msx2) (D). Control sections (E) were incubated without primary antibody. Arrows indicate calbindin-D_28k increased staining in epithelial rests of Malassez cells from Msx2^+/+ to Msx2^−/− incisors. Ab, ameloblasts; Od, odontoblasts; P, pulp; ERM, epithelial rests of Malassez. Top row, scale bar = 20 µm; bottom row, scale bar = 500 µm.