Table 1.
Gene Symbol | Primers | Annealing Temperature | Product Size (bp) | |
---|---|---|---|---|
Calbindin-D28k | Forward | 5′-ACGGAAGTGGTTACCTGGAA-3′ | 60 | 552 |
Reverse | 5′-CACACATTTTGATTCCCTGG-3′ | |||
GAPDH | Forward | 5′-AACCATAGGAAGGATACGGCTG-3′ | 058 | 450 |
Reverse | 5′-GGGGTGTGGTAACATCAGCAC-3′ | |||
Msx2 | Forward | 5′-CCTGAGGAAACACAAGACCA-3′ | 60 | 278 |
Reverse | 5′-AGTTGATAGGGAAGGGCAGA-3′ |