Fig. 3.

EL supplies cells with HDL-derived LPC.

A) LacZ- and EL-Ad infected HAEC were incubated with [¹⁴C]-PC labeled HDL in serum-free medium for 5 h. Following extensive washing, lipids were extracted from cell layers and separated by TLC. The signals corresponding to [¹⁴C]-LPC were visualized upon exposure of the TLC plates to a tritium screen (GE Healthcare) on the STORM imager.

B) HAEC were incubated with 1 μM [¹⁴C]-LPC in full medium containing 5% FCS in the absence or presence of indicated concentrations of thimerosal for 30 min, followed by extraction and analysis of [¹⁴C]-LPC and [¹⁴C]-PC by TLC as described in A).