FIGURE 5.

Mutations disrupting Nedd4-2 targeting site in hERG eliminate both Nedd4-2 and Cav3-mediated effects on hERG channels. A and B, effects of Nedd4-2 overexpression on the protein expressions (A) and the currents (B) of WT, Y1078A, or Δ1073 hERG channels. C and D, effects of Cav3 overexpression on the protein expressions (C) and the currents (D) of WT, Y1078A, or Δ1073 hERG channels. Stable HEK cell lines expressing WT, Y1078A, or Δ1073 mutant hERG channels were transfected with pcDNA3 (Ctl), Nedd4-2, or Cav3 plasmid. Experiments were performed 24 h after transfection. For Western blot analysis, each set of experiments was repeated four to seven times, respectively. For patch clamp experiments, the number of cells examined is shown above the bars. *, p < 0.05; **, p < 0.01 versus control.