FIGURE 6.

Interactions between Cav3 and Nedd4-2. A, Cav3 immunoprecipitates with Nedd4-2. Nedd4-2 or Cav3 was detected in proteins precipitated with an anti-Cav3 or anti-Nedd4-2 antibody, respectively, from whole cell proteins extracted from HEK 293 cells 24 h after Cav3 transfection (n = 5, for each set of experiments). B, Cav3 expression does not affect the whole cell expression level of Nedd4-2 but enhances the cell surface expression levels of Nedd4-2 (n = 4, **, p < 0.01 versus pcDNA3 (control)). C, overexpression of Cav3 enhances membrane localization of Nedd4-2 in HEK 293 cells. The expression of Cav3 and Nedd4-2 is demonstrated by showing their intensities in the line across both Cav3-nonexpressing and Cav3-expressing cells. Cav3 expression enhances the membrane expression of Nedd4-2.