FIGURE 3.

Mutations in HSV-1 UL8 where alanines have replaced single or clustered charged amino acids. Top, each segment represents 100 amino acids of the 750-amino acid HSV-1 UL8 protein. Each mutant, as defined in supplemental Table 1, is represented by a colored symbol. Some of the mutants are indicated. The color code indicates the phenotype based on experiments in this article as follows: green, wild type; red, lethal; yellow, impaired; pink, temperature-sensitive. Bottom, the amino acid replacements made in HSV-1 UL8 for mutants A23, A49, A52, and A53 are shown in boldface type. A ClustalW alignment of UL8 sequences from 41 different herpesviruses was made. A selection from this alignment, including evolutionarily diverged α-herpesviruses and all human herpesviruses, is shown. The following Refsequences and/or accession numbers have been used: BHV1, bovine herpesvirus 1, NC_001847; DEV, anatid herpesvirus 1, duck enteritis virus, NC_013036.1; EHV-1, equine herpesvirus 1, NC_001491; HSV-1, human herpesvirus 1, herpes simplex virus type 1, NC_001806; VZV, human herpesvirus 3, varicella zoster virus, NC_ 001348.