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GFP- and FLAG-tagged UL8 support DNA replication. Shown is an autoradiograph from a transient replication experiment using pORI(WT) and expression plasmids encoding HSV-1 replication proteins as indicated. The replication efficiencies for WT UL8, GFP-tagged, and FLAG-tagged UL8 when expressed as the ratio between replicated DNA and input DNA (see “Experimental Procedures”) were 1.0, 1.1, and 1.0.
